DNA Transfection: Calcium Phosphate Method by Munjin Kwon, Bonnie L. Firestein.
AbstractThe calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this are not yet defined; however, DNA–calcium precipitates internalized by the cells and efficiently expressed in almost all cell types. The cost-efficiency simplicity of allows use primary neuronal cultures, despite issues neurotoxicity. Here, we describe an optimized delivery plasmid dissociated cultures.Key wordsTransfectionCalcium phosphatePrimary culture