S9.4b Trimetallic Cu-Zn-Fe nanoparticles induced apoptosis and cell cycle arrest in multidrug-resistant Candida auris S9.4 Free oral presentations (late breaking), September 23, 2022, 4:45 PM – 6:15 PM by Hammad Alam, Aijaz Ahmad.
Abstract Background Candida species are opportunistic fungus that can cause serious infections, particularly in immunocompromised population. The number of fungal infections has increased steadily with being responsible for ˃ 70% these instances, hospitalized patients significant underlying conditions. Pharmacological resistance and the advent auris have elevated candidiasis to a major public health concern. is an emerging multidrug-resistant catastrophic bloodstream high fatality rates, medical issues. Antifungal study trimetallic nanoparticles (NPs) various types been studied as therapy option efficient safe control candidiasis. These NPs were highlighted environmentally friendly sustainable synthetic preparative possibilities. Objective: This work aimed synthesize characterize novel Cu-Zn-Fe determine their vitro antifungal activity mechanism action against isolates. Methods synthesis characterization was done by standard methods. capability determined calculating minimum inhibitory concentrations (MIC) fungicidal (MFC) following CLSI recommended guidelines. Susceptibility on planktonic cells biofilms further confirmed MuseTM cell count viability assay scanning electron microscopy (SEM) respectively. For insight mechanisms, apoptosis cycle arrest exploring different apoptotic markers analyzer. Results Characterizations Fourier-transform infrared spectroscopy (FTIR), diffuse reflectance UV-visible spectroscopy, X-ray diffraction (XRD), (SEM), transmission (TEM) successful biosynthesis NPs. MIC MFC values 12.5 25 μg/ml results reporting 45.5%, 13.5%, 1.8% when C. treated 1/2 MIC, 2MIC Cell analysis revealed 91.2% healthy developing untreated G0/G1 phase, whereas 5.2% 3.7% S phase G2/M In contrast, NP-treated observed be arrested 49.3% at 2MIC. To physiology death caused NPs, we investigated mitochondrial membrane potential (∆ψm), live having stable (∆ψm) showed loss (∆ψm). Another important parameter yeast release cytochrome C from mitochondria cytosol resulting decreased cytosolic levels. Both test causing auris. Conclusion (Cu-Zn-Fe) displayed strong auris, S-phase, which could linked DNA damage. Important suggested apotosis All findings suggest taken next level research development medications.
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