injury

Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion

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Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion by Deng-ming Wei, Guang-zhao Huang, Yi-gu Zhang, Guang-xun Rao.

Objective: To study effect of triptolide(TL) on neuronal apoptosis in cerebral tissue rat after ischemia-reperfusion. Method: Triptolide at dose 0.2 or 0.4 mg·kg -1was intr aperitoneally injected once a day for 4 d. The focal ischemia-rep erfusion model was established with thread embolism middle artery before trip tolide injection the fourth day. Neurological deficit score rats eval uated; and immunohistochemical techniques were used to count positive cells express MPO TUNEL tissue. Result: Compare d control group, neural function significantly impr oved, number infiltrate neutrophil cere bral remarkably reduced two TL-treated groups. Conclusio n: results suggested that TL can inhibit infiltration decrease degree [

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Biochemical Aspects of the Soybean Response to Herbivory Injury by the Brown Stink Bug Euschistus heros (Hemiptera: Pentatomidae)

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Biochemical Aspects of the Soybean Response to Herbivory Injury by the Brown Stink Bug Euschistus heros (Hemiptera: Pentatomidae) by Renata Velozo Timbó, Marcelo Hermes-Lima, Luciano P. Silva, Angela Mehta, Maria Carolina Blassioli-Moraes, Débora P. Paula.

Plant defense response is an elaborate biochemical process shown to depend on the plant genetic background and biological stressor. This work evaluated soybean foliar brown stink bug herbivory injury through analysis of redox metabolism proteomic 2DE profiles susceptible (BRS Silvania RR) resistant (IAC-100) varieties. The activity lipoxygenase-3, guaiacol peroxidase, catalase ascorbate peroxidase was monitored every 24 h up 96 h. In variety, caused increase in activities lipoxygenase 3 no change a decrease catalase. did not cause alteration any these enzymes. were after revealed have similar proportion (4-5%) differential protein expression both proteins, identified by mass spectrometry, variety related general stress responses, defense, fungal infections. However, profile Calvin cycle While showed adaptive changes stress-responsive circumvent

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Broad-Spectrum Matrix Metalloproteinase Inhibition Curbs Inflammation and Liver Injury but Aggravates Experimental Liver Fibrosis in Mice

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Broad-Spectrum Matrix Metalloproteinase Inhibition Curbs Inflammation and Liver Injury but Aggravates Experimental Liver Fibrosis in Mice by Vincent E de Meijer, Deanna Y. Sverdlov, Yury Popov, Hau D. Le, Jonathan A. Meisel, Vânia Nosé, Detlef Schuppan, Mark Puder.

Liver fibrosis is characterized by excessive synthesis of extracellular matrix proteins, which prevails over their enzymatic degradation, primarily metalloproteinases (MMPs). The effect pharmacological MMP inhibition on fibrogenesis, however, largely unexplored. Inflammation considered a prerequisite and important co-contributor to is, in part, mediated tumor necrosis factor (TNF)-alpha-converting enzyme (TACE). We hypothesized that treatment with broad-spectrum TACE-inhibitor (Marimastat) would ameliorate injury inflammation, leading decreased fibrogenesis during repeated hepatotoxin-induced liver injury.Liver was induced mice carbon tetrachloride (CCl4) administration, the received either Marimastat or vehicle twice daily. A single dose CCl4 administered investigate acute pretreated Marimastat, deficient Mmp9, both TNF-alpha receptors. quantified alanine aminotransferase (ALT) levels confirmed histology. Hepatic collagen determined as hydroxyproline, expression fibrolysis-related transcripts quantitative reverse-transcription polymerase chain reaction. Marimastat-treated animals demonstrated significantly attenuated inflammation but 25% increase deposition. Transcripts related were less upregulated compared vehicle-treated animals, while activity analysis revealed efficient pharmacologic MMP-inhibition fibrolysis following treatment. pre-treatment CCl4-administration, whereas Mmp9 no protection. Mice receptors exhibited an 80% reduction serum ALT, confirming hepatoprotective effects via TNF-signaling pathway.Inhibition TACE chronic administration counterbalanced any beneficial anti-inflammatory effect, resulting positive balance Since effective MMPs accelerates progression, inhibitors should be used caution patients diseases.

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Abdominal injury from sporting activities.

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Abdominal injury from sporting activities. by David Bergqvist, H Hedelin, G Karlsson, B Lindblad, Thomas Mätzsch.

The frequency of abdominal injury is rising. In an analysis 1,354 cases closed trauma sustained during a 30-year period (1950-1979) in Skaraborg County, Sweden, distinct increase was found the numbers associated with sporting activities. severity injuries caused by sports likewise showed increase. representation injured organs same order as total series injuries. An 136 sports-associated presented regard to cause and type injuries, diagnosis prognosis.

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[Injury of the anterior interosseous nerve in the differential diagnosis of the canalicular syndrome].

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[Injury of the anterior interosseous nerve in the differential diagnosis of the canalicular syndrome]. by Jurjević A, Milohanović S, Ledić P, Willheim K.

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Modifications of plasma enzyme activities after severe head injury; evaluation of prognosis using multivariate methods

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Modifications of plasma enzyme activities after severe head injury; evaluation of prognosis using multivariate methods by G. Jung, J. Morel, A. Bourguignat, G. Férard.

The aim of this study was to analyse the plasma variations four enzymatic activities (lactate dehydrogenase, aldolase, creatine kinase, aspartate aminotransferase) in 134 patients suffering from severe head injury. Enzymatic were assayed daily for 3 days after trauma. Means significantly different according their evolution (death or survival), except 48 h Multivariate analysis indicated that lactate dehydrogenase and aldolase levels useful order discriminate between potential survivors non-survivors. value multivariate traumatology is discussed.

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A Drug That Increases cGMP Blocks Thromboxane Induced Pulmonary Hypertension and Reduces Edema Formation in Oxidant Lung Injury

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A Drug That Increases cGMP Blocks Thromboxane Induced Pulmonary Hypertension and Reduces Edema Formation in Oxidant Lung Injury by M. H. JafriJr., Gail H. Gurtner.

Oxidants such as t-butyl-hydroperoxide (t-bu-OOH) cause vascular injury via lipid peroxidation and the consequent release of thromboxane peptide leukotrienes. It has been shown that agents which increase cAMP ameliorate lung (J. Appl Physiol. 62(1): 47–54, 1987). In this study, we sought to determine if cGMP may play a similar role. We investigated modulation t-bu-OOH induced by sodium nitroprusside (SNP) in isolated rabbit perfused with Krebs-Henseleit buffer ventilated 5% CO2 air. The perfusate was pretreated 1mM SNP or no pharmacological intervention for twenty minutes before serial one minute challenges t-bu-OOH. occurred ten intervals [t-bu-OOH] = 300μM first four challenges, 600 μM fifth challenge, 900 sixth challenge. untreated group, infusion produces an acute peak PPa, airway pressure, weight gain. treated lungs exhibited PPa 10.55 ±.64 vs. 30.86 ± 6.83 mm Hg (̄ SEM). mean pressure 9.43 ±.35 19.97 3.66 controls. Increases gain were significantly reduced treatment SNP:.2 ±.09 1.08 ±.56 grams/minute (p <.05). other experiments, left atrial preparation raised 5, 10, 15 attempt further characterize protective effect SNP. There significant difference at elevated between control. These findings suggest be due its action on microvascular pressure. (Supported PHS Grant HL35483.)KeywordsAirway PressureSodium NitroprussideLeft Atrial PressureRabbit LungYork Medical CollegeThese keywords added machine not authors. This process is experimental updated learning algorithm improves.

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Rotational laxity greater in patients with contralateral anterior cruciate ligament injury than healthy volunteers

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Rotational laxity greater in patients with contralateral anterior cruciate ligament injury than healthy volunteers by T. P. Branch, Jon E. Browne, John D. Campbell, Rainer Siebold, Howard Freedberg, Elizabeth A. Arendt, Frédéric Lavoie, Philippe Neyret, Cale A. Jacobs.

Rotational stability of the knee has been traditionally difficult to quantify, limiting ability orthopedic community determine potential role rotational laxity in etiology anterior cruciate ligament (ACL) injuries. The purposes this multicenter cohort study were evaluate reliability a robotic axial rotation measurement system, whether uninjured knees patients that had previous contralateral ACL reconstruction demonstrated different biomechanical characteristics than group healthy volunteers, and differ between male female non-injured limbs groups both volunteers with injury. Fourteen 79 unilateral injury participated study. Patients tested using computerized tibial system. Only normal (non-operated) data used for analysis. In order assess 10 daily over four consecutive days by examiners. compliance measures excellent (ICC = 0.97). significantly increased internal (20.6° vs. 11.4°, P < 0.001) reduced external (16.7° 26.6°, compared volunteers. Females rotation, as well males (P 0.05). Computer-assisted techniques may offer clinicians an accurate, reliable, non-invasive method select most appropriate preventative or surgical interventions laxity.

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