reperfusion

Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion

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Influence of triptolide on neuronal apoptosis in rat with cerebral injury after focal ischemia reperfusion by Deng-ming Wei, Guang-zhao Huang, Yi-gu Zhang, Guang-xun Rao.

Objective: To study effect of triptolide(TL) on neuronal apoptosis in cerebral tissue rat after ischemia-reperfusion. Method: Triptolide at dose 0.2 or 0.4 mg·kg -1was intr aperitoneally injected once a day for 4 d. The focal ischemia-rep erfusion model was established with thread embolism middle artery before trip tolide injection the fourth day. Neurological deficit score rats eval uated; and immunohistochemical techniques were used to count positive cells express MPO TUNEL tissue. Result: Compare d control group, neural function significantly impr oved, number infiltrate neutrophil cere bral remarkably reduced two TL-treated groups. Conclusio n: results suggested that TL can inhibit infiltration decrease degree [

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The effects of various fatty acids on action potential shortening during sequential periods of ischaemia and reperfusion

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The effects of various fatty acids on action potential shortening during sequential periods of ischaemia and reperfusion by J.C. Cowan, E M Williams.

Abstract Intracellular potentials were recorded from Langendorff-perfused guinea-pig hearts. All fatty acids studied (palmitate, linoleate, octanoate and acetate) potentiated action potential (AP) shortening in an experimental protocol involving sequential periods of coronary flow reduction reperfusion. Pyruvate acetoacetate did not share this effect. The dose relation the AP effect was case palmitate found to saturate at a relatively low palmitate: albumin molar ratio. Palmitate-induced potentiation ischaemic less marked sustained ischaemia than Palmitate acetate shown cause excerbation decline glycogen levels protocol. Ischaemic duration closely correlated with content low, but high levels. It seemed possible that part acid induced might be attributable depletion. In absence glycolytic substrate, shortening, whereas without degree associated stimulation exogenous glucose utilization, prevent stimulation.

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Prevention of reperfusion damage in working rat hearts by calcium antagonists and calmodulin antagonists

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Prevention of reperfusion damage in working rat hearts by calcium antagonists and calmodulin antagonists by Alan J. Higgins, Kenneth J. Blackburn.

The direct myocardial protection afforded by three structurally distinct calcium antagonists (0.1 micron nifedipine, 0.1 verapamil and 0.4 diltiazem), a calmodulin antagonist (20 W-7) was assessed in isolated working rat hearts subjected to 30 min global ischaemia followed reperfusion. At these concentrations, no drug-induced cardiac depression nor coronary vasodilatation observed prior ischaemia. All four agents improved recovery of function (assessed as total output) on reperfusion (by 49%, 29%, 64% 72% respectively, compared controls), attenuated the release lactate dehydrogenase 52%, 55%, 65% 66% respectively) inhibited intracellular 45Ca accumulation 42%, 35%, 49% 45% respectively). Despite increased tissue enzyme leakage reperfused hearts, [3H]inulin-impermeable space not decreased, suggesting specific changes membrane permeability rather than partial sarcolemmal rupture. Drug treatment did alter rate extent high-energy phosphate depletion during ischaemia, thus eliminating ATP preservation negative inotropy mechanisms for protective effects this system. Improved restoration flow obtained treated but we believe more likely be consequence vasodilatation. Thus, beneficial probably resulted from cellular viability. When given only phase, nifedipine W-7 were almost effective when before whereas diltiazem inactive. This highlights differences between various structural subclasses antagonists. Furthermore, efficacy antagonist, W-7, system suggests possible key role calmodulin-activated enzymes progression damage.

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Effect of gangliosides on tissue norepinephrine and Na/K-ATPase activity during cardiac hypoxia and reperfusion

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Effect of gangliosides on tissue norepinephrine and Na/K-ATPase activity during cardiac hypoxia and reperfusion by Maura Lodovici.

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Intraaortic balloon counterpulsation with and without reperfusion for myocardial infarction shock.

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Intraaortic balloon counterpulsation with and without reperfusion for myocardial infarction shock. by Marcus A. DeWood, R. N. Notske, Gerald R. Hensley, J. P. Shields, William P. O’Grady, J Spores, M Goldman, J H Ganji.

Forty patients were treated for cardiogenic shock secondary to acute myocardial infarction. Twenty-one (group 1) with intraaortic balloon counterpulsation and 19 2) coronary artery bypass grafting. The groups similar in age, incidence of previous infarction, initial hemodynamics anatomy. in-hospital mortality between group 1 (52.4%) 2 (42.1%) was not significantly different. difference long-term substantially different (71.4% vs 47.3%). subset (n = 12) that underwent reperfusion within 16 hours from the onset symptoms infarction had a lower (25.0%) than 7) operation more 18 after (71.4%). operated on (25.0% 71.4%, p less 0.03). data suggest is effective when carried out early. Patients who develop appear benefit most if alone.

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Differing time courses between $Delta;lactate and mitochondrial respiration during coronary occlusion and after reperfusion in canine hearts

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Differing time courses between $Delta;lactate and mitochondrial respiration during coronary occlusion and after reperfusion in canine hearts by Kazumi Taki, Yoshihiro Hanaki, Satoru Sugiyama, T. Kato, S. Suzuki, Takayuki Ozawa.

The present study was designed to clarify whether or not a difference between arterial and venous lactate (Δlactate) levels is useful for evaluation of mitochondrial function in ischemia-reperfused myocardium. In the first experiment, 12 dogs were divided into 2 groups: 10-min occlusion left anterior descending coronary artery (LAD) followed by reperfusion, 30-min 40-min performed. femoral great cardiac vein measured enzymatically. ΔLactate reversed immediately after occlusion. Ten min 20 required recovery Δlactate 10-min-occlusion with 10-min-reperfusion, 30-min-occlusion 40-min-reperfusion groups, respectively. second 36 6 LAD; reperfusion; occlusion; 10-, 20-, reperfusion Mitochondria from normal occluded reperfused areas prepared, respiratory mitochondria polarographically. No significant decreases observed 10-min-occlusion, 10-min-reperfusion groups. On other hand, impaired improved 10- 20-min reperfusion. Significant That is, differing time courses observed.

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Peroxynitrite may play a role in ischemia reperfusion injury in human myocardium

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Peroxynitrite may play a role in ischemia reperfusion injury in human myocardium by Yoshitaka Hayashi, Yoshiki Sawa, Motonobu Nishimura, Akira Amemiya, Toshihiro Ohata, Hideki Ueda, Yoshihisa Naka, Taichi Sakaguchi, Shigeaki Ohtake, Hikaru Matsuda.

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The second generation of Carolina Rinse, solution II, improves graft survival following orthotopic liver transplantation in the rat by preventing reperfusion injury

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The second generation of Carolina Rinse, solution II, improves graft survival following orthotopic liver transplantation in the rat by preventing reperfusion injury by W. Gao, J.J. Lemasters, R.G. Thurman.

Carolina Rinse solution was designed to minimize reperfusion injury following orthotopic liver transplantation. blocks reperfusion-induced endothelial cell killing, diminishes postoperative enzyme release and improves survival dramatically. Adenosine mildly acidotic pH were identified as key components. Here we report results with a simplified formulation, H, which contains extracellular inorganic ions similar Ringer’s solution, adenosine, well antioxidants radical scavengers (allopurinol, glutathione desferrioxamine). In this study, 44 rat livers explanted stored for 12 h in University of Wisconsin (UW) cold storage (non-survival conditions). Control rinsed 15 ml just prior completion implantation surgery. control group, average 30-day poor (8%). However, increased around 60% when grafts II. Survival not improved significantly by rinsing the graft containing adenosine omitted (about 30%). Peak SGOT values nearly 3000 U/l, measured 1–3 days postoperatively rinse decreased 4- 5-fold both II antioxidants. On other hand, addition (around 60%) but did decrease elevation serum enzymes significantly. Thus, it appears that necessary optimal whereas needed prevent transplanted graft. These data consistent hypothesis at least two mechanisms, one involving second non-hepatic, are responsible post-transplant patho-physiology. also reduced 2- 3-fold under conditions (e. g., 8 UW solution). This study demonstrated convincingly very simple II, minimized

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Role of early reperfusion in the induction of adhesion molecules and cytokines in previously ischemic myocardium

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Role of early reperfusion in the induction of adhesion molecules and cytokines in previously ischemic myocardium by Gilbert L. Kukielka, Keith A. Youker, Lloyd H. Michael, Ajith G. Kumar, Christie M. Ballantyne, C. Wayne Smith, Mark L. Entman.

Our studies in vitro demonstrate that neutrophil mediated injury of isolated cardiac myocytes requires the presence ICAM1 on surface myocyte and CD11b/CD18 activation neutrophil. In post-ischemic lymph, there is rapid appearance C5a activity during first hours reperfusion. Interleukin-6 present throughout 72 h reperfusion sufficient to induce ICAM-1 myocyte. situ hybridization suggest mRNA found viable myocardial cells edge infarction within 1 protein expression seen after 6 reperfusion, increases thereafter. Non-ischemic tissue demonstrates no early induction or cells. our most recent experiments, we have determined an absolute requirement for previously ischemic To further assess this, cloned sequenced a canine interleukin-6 (IL-6) cDNA. The data IL-6 also dependent as it could be demonstrated same reperfused segments which was found. Peak occurred much earlier than mRNA. Similar experiments were then performed with molecular probe interleukin-8 (IL-8). This chemokine potent stimulant has higher degree specificity neutrophils classic chemoattractants such C5a. results similar pattern occurs hour markedly increased by relationship cytokine discussed. (Mol Cell Biochem 147: 5–12, 1995)Key wordsmyocardial injurymyocardial infarctioninterleukin-6interleukin-8cell adhesion moleculesneutrophils

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