A dual role for microglia in promoting tissue inhibitor of metalloproteinase (TIMP) expression in glial cells in response to neuroinflammatory stimuli by Jennifer V. Welser-Alves, Stephen J. Crocker, Richard Milner.
Abstract Background By neutralizing the effect of matrix metalloproteinases (MMPs), tissue inhibitors (TIMPs) play a critical role in maintaining proteolysis balance. As major reactive glial cell types central nervous system (CNS), microglia and astrocytes fundamental roles mediating breakdown repair. such, it is important to define TIMP expression profile these cells, as well mechanisms regulation by neuroinflammatory stimuli. Methods Primary mixed cultures (MGC), pure microglia, were used this study. To study astrocytes, we employed recently described astrocyte culture system, which has advantage totally lacking microglia. The three different treated with lipopolysaccharide (LPS) or individual cytokines, supernatants assayed for TIMP-1 TIMP-2 protein western blot. Results LPS induced MGC, but not microglial cultures. When cytokines IL-1β, IFN-γ, TNF TGF-β1, only IL-1β expression. Significantly, was restored LPS-treated after addition conditioned medium taken from LPS-activated (MG-CM). Furthermore, lost depletion MG-CM. contrast, constitutively expressed whereas exposure serum. Conclusions Taken together, results demonstrate an concept interactions, showing that regulating TIMPs, identify playing key microglial-astrocyte communication.
Read A dual role for microglia in promoting tissue inhibitor of metalloproteinase (TIMP) expression in glial cells in response to neuroinflammatory stimuli on OA.mg