The Biological Activity of Natural and Mutant Ptα Alleles by Deena L. Gibbons, Nataki C. Douglas, Domingo F. Barber, Qiang Liu, Renee Sullo, Liping Geng, Fehling Hj, Harald von Boehmer, Adrian Hayday.
beta selection is a major checkpoint in early thymocyte differentiation, mediated by successful expression of the pre-T cell receptor (TCR) comprising TCRbeta chain, CD3 proteins, and surrogate TCRalpha pTalpha. The mechanism action pre-TCR unresolved. In humans mice, pTalpha gene encodes two RNAs, pTalpha(a), substantially truncated form, pTalpha(b). This study shows that both are biologically active their capacity to rescue multiple defects pTalpha(-/-) mice. Further alleles include one lacks ectodomain much long cytoplasmic tail (which unique among antigen chains), another which substituted with short TCR Calpha. Thus, very little chain required for function. These data support hypothesis primary role stabilize pre-TCR, conserved structure probably plays critical regulatory role.
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