THE NORMAL FLORA OF THE BOVINE RUMEN II. QUANTITATIVE BACTERIOLOGICAL STUDIES by Marion K. Wilson, C. A. E. Briggs.
SUMMARY: A simple medium and dilution count technique was evolved for determining the viable of rumen bacteria. Material from three fistulated cows examined population found to vary within range 107-1012/g; most frequently occurring values were 108-1010/g (94.0% 350 counts). Observations made homogeneity contents, variations in with time after sampling between cows, variation day day, hour hour, before feeding on different diets. The count, even gross changes diet, never greater than those a single animal fixed diet. Microscopic examinations showed Gram-positive cocci rods be types bacteria, Gram-negative organisms coli-aerogenes type being present only low numbers.
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